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Partially purified cell membrane antigens from fresh leukaemia cells. (Human).
Our Abpromise guarantee covers the use of ab11414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent dilution. Use 10ul of the neat dilution to label 106 cells in 100ul.|
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 75 kDa. CD105 is a glycoprotein homodimer of 95kD subunits.|
|IHC-P||Use at an assay dependent dilution. PubMed: 21612554|
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
IHC - Wholemount of rat brain postnatal sections labelling CD105 with ab11414. Sample was incubated with primary antibody (1/200) for 12 hours at 4°C. An Alexa Flour® 488-conjugated rabbit anti-mouse IgG monoclonal (1/2000) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"