Overview

  • Product nameCD105 (Endoglin) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    12.35 pg/ml - 3000 pg/ml
  • Recovery

    > 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.92 87% - 110%
    Serum 120.1 111% - 130%
    Plasma 116.7 94% - 132%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD105 (Endoglin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD105 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD105 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD105 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD105 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD105 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.
  • Alternative names
    • AI528660
    • AI662476
    • CD 105
    • CD105 antigen
    • Cell surface MJ7/18 antigen
    • END
    • Endoglin
    • ENG
    • FLJ41744
    • HHT1
    • ORW
    • ORW1
    • Osler Rendu Weber syndrome 1
    • OTTMUSP00000012838
    • OTTMUSP00000012839
    • RP11 228B15.2
    • RP23 17P12.4
    • S endoglin
    • Transmembrane glycoprotein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD105 (Endoglin) Human ELISA Kit images

  • Representative Standard Curve using ab100507.

Protocols

References for CD105 (Endoglin) Human ELISA Kit (ab100507)

ab100507 has not yet been referenced specifically in any publications.

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ab100507 CD105 human ELISA kit has been validated with plasma and is compatible with EDTA, heparin, and citrate anti-coagulants unless otherwise stated in the Introduction section of the manual. Any one from the ab...

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Optimal dilution of the sample will usually need to be optimized by the end user. In this case, I would appreciate if you could let me know what type of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"