Anti-CD109 antibody [B-E47] (Phycoerythrin) (ab27343)

Overview

  • Product name
    Anti-CD109 antibody [B-E47] (Phycoerythrin)
    See all CD109 primary antibodies
  • Description
    Mouse monoclonal [B-E47] to CD109 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) -Eahy 926 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 5% BSA, PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Clonality
    Monoclonal
  • Clone number
    B-E47
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Modulates negatively TGFB1 signaling in keratinocytes.
  • Tissue specificity
    Widely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
  • Sequence similarities
    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Post-translational
    modifications
    N-glycosylated.
    2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 KDa TGF beta 1 binding protein antibody
    • 150 KDa TGF-beta-1-binding protein antibody
    • 7D1 antibody
    • 8A3 antibody
    • Activated T cell marker CD109 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody
    • CD 109 antibody
    • CD109 antibody
    • CD109 antigen antibody
    • CD109_HUMAN antibody
    • CPAMD7 antibody
    • DKFZp762L1111 antibody
    • E123 antibody
    • FLJ38569 antibody
    • FLJ41966 antibody
    • Gov system alloantigens on platelets antibody
    • p180 antibody
    • Platelet specific Gov antigen antibody
    • Platelet-specific Gov antigen antibody
    • r150 antibody
    • RP11 525G3.1 antibody
    see all

References

ab27343 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen of immunized rat
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Application
Flow Cytometry
Sample
Human Cell (from buffy coat)
Specification
from buffy coat
Preparation
Cell harvesting/tissue preparation method: after ficoll extraction
Sample buffer: PBS/BSA 1%
Fixation
none
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Fixation
none
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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