Anti-CD109 antibody [B-E47] (Phycoerythrin) (ab27343)

Overview

  • Product nameAnti-CD109 antibody [B-E47] (Phycoerythrin)
    See all CD109 primary antibodies
  • Description
    Mouse monoclonal [B-E47] to CD109 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) -Eahy 926 cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 5% BSA, PBS
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • ClonalityMonoclonal
  • Clone numberB-E47
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionModulates negatively TGFB1 signaling in keratinocytes.
  • Tissue specificityWidely expressed with high level in uterus, aorta, heart, lung, trachea, placenta and in fetal heart, kidney, liver, spleen and lung. Expressed by CD34(+) acute myeloid leukemia cell lines, T-cell lines, activated T-lymphoblasts, endothelial cells and activated platelets. Isoform 5 is expressed in placenta. Isoform 1 is expressed in keratinocytes and placenta.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Post-translational
    modifications
    N-glycosylated.
    2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 150 KDa TGF beta 1 binding protein antibody
    • 150 KDa TGF-beta-1-binding protein antibody
    • 7D1 antibody
    • 8A3 antibody
    • Activated T cell marker CD109 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 antibody
    • CD 109 antibody
    • CD109 antibody
    • CD109 antigen antibody
    • CD109_HUMAN antibody
    • CPAMD7 antibody
    • DKFZp762L1111 antibody
    • E123 antibody
    • FLJ38569 antibody
    • FLJ41966 antibody
    • Gov system alloantigens on platelets antibody
    • p180 antibody
    • Platelet specific Gov antigen antibody
    • Platelet-specific Gov antigen antibody
    • r150 antibody
    • RP11 525G3.1 antibody
    see all

References for Anti-CD109 antibody [B-E47] (Phycoerythrin) (ab27343)

ab27343 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen of immunized rat
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Application Flow Cytometry
Sample Human Cell (from buffy coat)
Specification from buffy coat
Preparation Cell harvesting/tissue preparation method: after ficoll extraction
Sample buffer: PBS/BSA 1%
Fixation none
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Fixation none
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"