Overview

  • Product nameAnti-CD11a antibody [121/7]
    See all CD11a primary antibodies
  • Description
    Rat monoclonal [121/7] to CD11a
  • SpecificityThis antibody does not react with Human Jurkat cells in FACS applications.
  • Tested applicationsIP, Blocking, WB, Neutralising, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Mouse CD11a.

Properties

Applications

Our Abpromise guarantee covers the use of ab119356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.
Neutralising Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
  • Tissue specificityLeukocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • Antigen CD11A antibody
    • CD 11a antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD11a antigen antibody
    • Integrin Alpha L antibody
    • Integrin alpha-L antibody
    • Integrin gene promoter antibody
    • Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody
    • ITAL_HUMAN antibody
    • Itgal antibody
    • ITGAL protein antibody
    • Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody
    • Leukocyte function associated molecule 1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA 1A antibody
    • LFA-1A antibody
    • LFA1A antibody
    • Ly15 antibody
    • Ly21 antibody
    • Lymphocyte function associated antigen 1 antibody
    • Lymphocyte Function Associated Antigen Type 1 alpha antibody
    • p180 antibody
    see all

Anti-CD11a antibody [121/7] images

  • Flow cytometry analysis of CD11a in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119356) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.

References for Anti-CD11a antibody [121/7] (ab119356)

This product has been referenced in:
  • Champe M  et al. Monoclonal antibodies that block the activity of leukocyte function-associated antigen 1 recognize three discrete epitopes in the inserted domain of CD11a. J Biol Chem 270:1388-94 (1995). Read more (PubMed: 7530713) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"