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Full length native protein (purified) corresponding to Mouse CD11a.
Our Abpromise guarantee covers the use of ab119356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 128 kDa.|
|Neutralising||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. Ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|Inhibition Assay||Use at an assay dependent concentration.|
Flow cytometry analysis of CD11a in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119356) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"