The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Sequence similaritiesBelongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
ab34198, Using 10ul of neat antibody, Staining of human peripheral blood lymphocytes by Flow Cytometry.
References for Anti-CD11a antibody  (FITC) (ab34198)
This product has been referenced in:
Pelletier M & Girard D Interleukin-15 increases neutrophil adhesion onto human respiratory epithelial A549 cells and attracts neutrophils in vivo. Clin Exp Immunol141:315-25 (2005).
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