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Tissue/ cell preparation (Human). Peripheral blood lymphocytes.
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. We would recommend antibody ab52895 as a replacement.
Our Abpromise guarantee covers the use of ab3981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration. PubMed: 23039186|
|Flow Cyt||Use a concentration of 1 µg/ml.|
|Functional Studies||Use at an assay dependent concentration. The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 alpha subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1.|
|IP||Use at an assay dependent concentration.|