The monoclonal antibody is directed against the CD11a antigen, located on the alpha L chain of LFA1 complex, which is expressed on mature immunocompetent lymphocytes and their neoplastic counterparts, granulocytes and monocytes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab126030-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
ab123627, at 5 µl/106 cells, staining CD11a in normal Human peripheral blood cells by Flow Cytometry. Cells in the leukocyte gate were used for analysis.Blue histogram represents Monocytes cells, yellow represents lymphocytes and red represent Monocytes