The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19071955
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Predominantly expressed in monocytes and granulocytes.
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
ab62817 (2µg/ml) staining of paraffin embedded Human Brain shows staining of microglia following steamed antigen retrieval with Tris/EDTA buffer pH 9 and HRP-staining.
Immunohistochemistry (Frozen sections) - Anti-CD11b antibody (ab62817)This image is courtesy of an anonymous Abreview
ab62817 staining CD11b in mouse retina tissue sections by Immunohistochemistry (Frozen sections).
Tissue was fixed with paraformaldehyde and blocked with 3% BSA at room temperature for 40 minutes. Samples were incubated with primary antibody (1/200 in 1% BSA) for 24 hours at 4°C. An AlexaFluor® 488-conjugated donkey anti-goat polyclonal (1/150) was used as the secondary antibody.