Overview

  • Product nameAnti-CD11b antibody
    See all CD11b primary antibodies
  • Description
    Rabbit polyclonal to CD11b
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide within Mouse CD11b aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: P05555

  • Positive control
    • WB: RAW 264.7 whole cell lysate.

Applications

Our Abpromise guarantee covers the use of ab75476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 2 µg/ml. Detects a band of approximately 160 kDa.

We have mixed reports of results with human samples. Some lots works some doesn't. In case of negative results with human samples, please use mouse RAW 264.7 cell lysates as positive control.

Target

  • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody images

  • Anti-CD11b antibody (ab75476) at 2 µg/ml + RAW 264.7 whole cell lysate

    Observed band size : ~160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CD11b antibody (ab75476)

This product has been referenced in:
  • Her Z  et al. Loss of TLR3 aggravates CHIKV replication and pathology due to an altered virus-specific neutralizing antibody response. EMBO Mol Med 7:24-41 (2015). IHC-P ; Mouse . Read more (PubMed: 25452586) »
  • von Einem B  et al. The Golgi-Localized ?-Ear-Containing ARF-Binding (GGA) Proteins Alter Amyloid-ß Precursor Protein (APP) Processing through Interaction of Their GAE Domain with the Beta-Site APP Cleaving Enzyme 1 (BACE1). PLoS One 10:e0129047 (2015). WB, ICC . Read more (PubMed: 26053850) »

See all 26 Publications for this product

Product Wall

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
Permeabilization No
Specification Spleen
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse oral tumor cells (MOC-2 obtained from Uppalu)
Permeabilization No
Specification Mouse oral tumor cells (MOC-2 obtained from Uppalu
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Apr 09 2015

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cell from bone marrow)
Specification Cell from bone marrow
Gating Strategy Alive cells (DAPI-), blood cells exluded
Preparation Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

Submitted Feb 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step normal horse blocking serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 24°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: trypsin (1:3)
Sample Rat Tissue sections (alveolar bone)
Specification alveolar bone
Permeabilization No
Fixative Formaldehyde
Username

Ms. Dong-Eun Lee

Verified customer

Submitted Nov 14 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step None
Permeabilization No
Specification spleen
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Methanol
Username

Ms. CM ou

Verified customer

Submitted Jul 23 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain, cerebral ischemia, 12´m cryo sections)
Specification brain, cerebral ischemia, 12´m cryo sections
Blocking step 5 % serum, 0,25% Triton-X-100, in TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Mrs. Birte Könnecke

Verified customer

Submitted Apr 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver Teratoma)
Specification Liver Teratoma
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Dr. Shashank Ganatra

Verified customer

Submitted Oct 15 2012

I am very pleased to hear you would like to test ab75476 in IHC-Fr. This code will give you a discount off your next order before the expiration date. You can use it to purchase an IHC-Fr tested antibody, like ab8878. To redeem this offer, please submi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: AR-10 from Biogenex
Blocking step Power Block from Biogenex + 2% serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: rt°C
Username

Dr. Laura Dutca

Verified customer

Submitted Sep 28 2012

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"