Overview

  • Product nameAnti-CD11b antibody [CD11b]
    See all CD11b primary antibodies
  • Description
    Mouse monoclonal [CD11b] to CD11b
  • Tested applicationsSuitable for: ICC/IF, IHC - Wholemount, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD11b. Immunogen is rat neutrophils.

Applications

Our Abpromise guarantee covers the use of ab8879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [CD11b] images

  • IHC - Wholemount of rat retina tissue labelling CD11b (white) with ab8879. Sample was incubated with primary antibody (1/100 in PBS) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody. Blue - DAPI.

    See Abreview

  • ab8879 staining CD11b in mixed rat spinal cord cultures on astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were live stained. Samples were incubated with primary antibody (1/200 in DMEM) for 20 minutes. An Alexa Fluor®488-conjugated Rat anti-rabbit IgG1 monoclonal (1/600) was used as the secondary antibody.

    See Abreview

  • NR8383 cells, rat macrophage cells, were used for a flow cytometry analysis with ab8879 (CD11b) antibody that binds the rat Mac-1 alpha subunit (CD11b). Cells were treated with ab8879 at a 1:50 dilution and subsequently with anti-murine IgG FITC. A 94% shift is seen as compared to the non-treated control.

References for Anti-CD11b antibody [CD11b] (ab8879)

This product has been referenced in:
  • Liu J  et al. HIV-1 Tat protein increases microglial outward K(+) current and resultant neurotoxic activity. PLoS One 8:e64904 (2013). ICC/IF ; Rat . Read more (PubMed: 23738010) »
  • Rui YF  et al. Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy. Stem Cells Dev 22:1076-85 (2013). Flow Cyt ; Rat . Read more (PubMed: 23106341) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Victor Maximov

Verified customer

Submitted May 28 2014

Application IHC - Wholemount
Sample Rat Tissue (retina whole mount)
Specification retina whole mount
Username

Abcam user community

Verified customer

Submitted May 06 2014

I am very pleased to hear you would like to accept our offer and test ab8879 in IHC-Fr on rat. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr...

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Thank you for your enquiry.

I can confirm that this antibody (ab8879: Anti-CD11b) is not affinity purified but ascitic fluid. The concentration of our current stock is 1.86 mg/ml and the unit size (mass) is 100 µg; the volume size depen...

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Thank you for your enquiry and your interest in our products.

I can confirm that this antibody (ab52478: Anti-CD11b antibody [EP1345Y]) is raised in rabbit; it has been tested for cross-reactivity only with mouse and human CD11b. It has not b...

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Thank you for contacting Abcam Attached please find the two MSDS forms you requested. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

Thank you for contacting us. According to the literature, both CD11b and CD11c are good microglial (neuronal macrophage) markers: http://www.jneuroinflammation.com/content/4/1/26 Ab1211 has successfully been tested in mouse and rat, and...

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Thank you for your inquiry. I can confirm that CD11b is widely used as microglia marker and therefore I can recommend to use ab8879 (Anti-CD11b antibody [CD11b]). This antibody is tested and guaranteed for rat samples and the following appl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (mixed rat spinal cord cultures on astrocytes)
Specification mixed rat spinal cord cultures on astrocytes
Fixative live staining
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 16 2010

Thank you for your phone call. I'm sorry to hear that this antibody did not work out for you in Western blotting or in IHC. This antibody has only been tested for application in IP and FACS so far. We greatly appreciate your feedback and I suggest that...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"