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Tissue, cells or virus corresponding to Rat CD11b. Immunogen is rat neutrophils.
Our Abpromise guarantee covers the use of ab8879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
NR8383, rat macrophage cells, were used for a flow cytometry analysis with ab8879 (CD11b) antibody that binds the rat Mac-1 alpha subunit (CD11b). Cells were treated with ab8879 at a 1:50 dilution and subsequently with anti-murine IgG FITC. A 94% shift is seen as compared to the non-treated control.
IHC - Wholemount of rat retina tissue labelling CD11b (white) with ab8879. Sample was incubated with primary antibody (1/100 in PBS) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody. Blue - DAPI.
ab8879 staining CD11b in mixed rat spinal cord cultures on astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were live stained. Samples were incubated with primary antibody (1/200 in DMEM) for 20 minutes. An Alexa Fluor®488-conjugated Rat anti-rabbit IgG1 monoclonal (1/600) was used as the secondary antibody.