Overview

  • Product name
    Anti-CD11b antibody [CD11b]
    See all CD11b primary antibodies
  • Description
    Mouse monoclonal [CD11b] to CD11b
  • Tested applications
    Suitable for: ICC/IF, IHC - Wholemount, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD11b. Immunogen is rat neutrophils.

  • Positive control
    • Flow Cyt: NR8383, rat macrophage cells.

Applications

Our Abpromise guarantee covers the use of ab8879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease
    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [CD11b] images

  • NR8383, rat macrophage cells, were used for a flow cytometry analysis with ab8879 (CD11b) antibody that binds the rat Mac-1 alpha subunit (CD11b). Cells were treated with ab8879 at a 1:50 dilution and subsequently with anti-murine IgG FITC. A 94% shift is seen as compared to the non-treated control.

  • IHC - Wholemount of rat retina tissue labelling CD11b (white) with ab8879. Sample was incubated with primary antibody (1/100 in PBS) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody. Blue - DAPI.

    See Abreview

  • ab8879 staining CD11b in mixed rat spinal cord cultures on astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were live stained. Samples were incubated with primary antibody (1/200 in DMEM) for 20 minutes. An Alexa Fluor®488-conjugated Rat anti-rabbit IgG1 monoclonal (1/600) was used as the secondary antibody.

    See Abreview

References for Anti-CD11b antibody [CD11b] (ab8879)

This product has been referenced in:
  • Liu J  et al. HIV-1 Tat protein increases microglial outward K(+) current and resultant neurotoxic activity. PLoS One 8:e64904 (2013). ICC/IF ; Rat . Read more (PubMed: 23738010) »
  • Rui YF  et al. Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy. Stem Cells Dev 22:1076-85 (2013). Flow Cyt ; Rat . Read more (PubMed: 23106341) »

See all 9 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Victor Maximov

Verified customer

Submitted May 28 2014

Application
IHC - Wholemount
Sample
Rat Tissue (retina whole mount)
Specification
retina whole mount
Username

Abcam user community

Verified customer

Submitted May 06 2014

According to current literature, both CD11b and CD11c are suitable markers for microglia (neuronal macrophages).

Please see below for a reference:
http://www.jneuroinflammation.com/content/4/1/26

However, please note that...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (mixed rat spinal cord cultures on astrocytes)
Specification
mixed rat spinal cord cultures on astrocytes
Fixative
live staining
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Apr 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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