Overview

  • Product nameAnti-CD11b antibody [EP1345Y]See all CD11b primary antibodies ...
  • Description
    Rabbit monoclonal [EP1345Y] to CD11b
  • SpecificityTesting of Mouse and Rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any Rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
  • Tested applicationsICC/IF, IHC-FoFr, WB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C terminus of human CD11b

  • Positive control
    • IHC-P: Human spleen tissue WB: TF1 lysate
  • General notesProduced under U.S. Patent No. 5,675,063.

Applications

Our Abpromise guarantee covers the use of ab52478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24505439
IHC-FoFr Use at an assay dependent concentration.
WB 1/20000 - 1/50000. Predicted molecular weight: 128 kDa.
IP 1/80.
ICC 1/100 - 1/250.
Flow Cyt 1/20 - 1/50.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b antigen antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC 1 alpha subunit antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Cell Surface Glycoprotein MAC1 Alpha Subunit antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 Alpha Chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha M complement component 3 receptor 3 subunit antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte Adhesion Receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil Adherence Receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [EP1345Y] images

  • Anti-CD11b antibody [EP1345Y] (ab52478) at 1/20000 dilution + TF1 lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 128 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human spleen using ab52478 at a dilution of 1/100.
  • Overlay histogram showing RAW 264.7 cells stained with ab52478 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52478, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • All lanes : Anti-CD11b antibody [EP1345Y] (ab52478) at 1/5000 dilution

    Lane 1 : THP-1 monocytes, untreated
    Lane 2 : THP-1 monocytes, +10 ng/ml LPS
    Lane 3 : THP-1 macrophages, untreated
    Lane 4 : THP-1 macrophages, +10 ng/ml LPS

    Lysates/proteins at 40 µg per lane.

    Secondary
    IRDye®680-conjugated donkey anti-rabbit IgG
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-CD11b antibody [EP1345Y] (ab52478)

This product has been referenced in:
  • Di Meo S  et al. Interleukin-30 expression in prostate cancer and its draining lymph nodes correlates with advanced grade and stage. Clin Cancer Res 20:585-94 (2014). Human . Read more (PubMed: 24277453) »
  • Martin PT  et al. A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle. PLoS One 9:e88226 (2014). ICC/IF ; Human . Read more (PubMed: 24505439) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 3% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Merci de nous avoir contactés.

J’ai eu une réponse du laboratoire. Pour répondre a votre question concernant l’image d’IHC-P sur la fiche technique d’ab52478, un blocage de 10% sérum en PBS ...

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Suite à notre conversation téléphonique, j’ai contacté notre laboratoire pour leur demander le protocole qu'ils ont utilisé pour faire IHC-P sur notre fiche technique. J'attends leur réponse et je vous co...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 (human monocytic leukemia))
Loading amount 40 µg
Specification THP-1 (human monocytic leukemia)
Treatment 200 nM PMA for 72 hours (THP-1 macrophages)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2012

Thank you for contacting us.

This product has been tested by us in Flow Cytometry in RAW 264.7 cells. We have added this data to the datasheet. In regards to IHC-P, while this product is covered by our Abpromise guarantee in IHC-P in mouse...

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Gracias por tu respuesta.

Si en lugar de recibir ab27537 os es más conveniente recibir otro anticuerpo que haya sido testado en ratón mediante WB, no habría ningún problema.

De hecho yo recomendarí...

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Thank you for taking the time to contact us. I am sorry to hearthe customer has some concerns regarding the results from this antibody.

In answer to your question, the dilution for optimal resultswill often require individual optimization by...

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Thank you for your enquiry and your interest in our products.

I can confirm that this antibody (ab52478: Anti-CD11b antibody [EP1345Y]) is raised in rabbit; it has been tested for cross-reactivity only with mouse and human CD11b. It has not b...

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Merci de votre intérêt pour les anti-CD11b d'Abcam.

Anotre connaissance :
1) l'anticorps ab62817n’a pas encore été testéchez la souris (90% d'homologie entre l'immunogène et le CD11b de la s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"