• Product nameAnti-CD11b antibody [EP1345Y]
    See all CD11b primary antibodies
  • Description
    Rabbit monoclonal [EP1345Y] to CD11b
  • SpecificityTesting of Mouse and Rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any Rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C terminus of human CD11b

  • Positive control
    • IHC-P: Human spleen tissue WB: TF1 lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Our Abpromise guarantee covers the use of ab52478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-FoFr Use at an assay dependent concentration.
WB 1/20000 - 1/50000. Predicted molecular weight: 128 kDa.
IP 1/80.
Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [EP1345Y] images

  • IHC image of CD11b staining in a formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemical analysis of paraffin-embedded human spleen using ab52478 at a dilution of 1/100.
  • Overlay histogram showing RAW 264.7 cells stained with ab52478 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52478, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Anti-CD11b antibody [EP1345Y] (ab52478) at 1/20000 dilution + TF1 lysate at 10 µg

    goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 128 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD11b antibody [EP1345Y] (ab52478) at 1/5000 dilution

    Lane 1 : THP-1 monocytes, untreated
    Lane 2 : THP-1 monocytes, +10 ng/ml LPS
    Lane 3 : THP-1 macrophages, untreated
    Lane 4 : THP-1 macrophages, +10 ng/ml LPS

    Lysates/proteins at 40 µg per lane.

    IRDye®680-conjugated donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    Exposure time : 4 minutes

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 21°C.

    See Abreview

References for Anti-CD11b antibody [EP1345Y] (ab52478)

This product has been referenced in:
  • Yi L  et al. Involvement of calreticulin in cell proliferation, invasion and differentiation in diallyl disulfide-treated HL-60 cells. Oncol Lett 12:1861-1867 (2016). Human . Read more (PubMed: 27588133) »
  • Burm SM  et al. ATP-induced IL-1ß secretion is selectively impaired in microglia as compared to hematopoietic macrophages. Glia 64:2231-2246 (2016). Read more (PubMed: 27641912) »

See all 17 Publications for this product

Product Wall

Merci de votre intérêt pour les anti-CD11b d'Abcam.

Anotre connaissance :
1) l'anticorps ab62817n’a pas encore été testéchez la souris (90% d'homologie entre l'immunogène et le CD11b de la s...

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Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification THP-1
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Mar 27 2017

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human cells grown on interphase co-culture)
Permeabilization No
Specification human cells grown on interphase co-culture
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted May 24 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 3% Triton X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 08 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Merci de nous avoir contactés.

J’ai eu une réponse du laboratoire. Pour répondre a votre question concernant l’image d’IHC-P sur la fiche technique d’ab52478, un blocage de 10% sérum en PBS ...

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Suite à notre conversation téléphonique, j’ai contacté notre laboratoire pour leur demander le protocole qu'ils ont utilisé pour faire IHC-P sur notre fiche technique. J'attends leur réponse et je vous co...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 (human monocytic leukemia))
Loading amount 40 µg
Specification THP-1 (human monocytic leukemia)
Treatment 200 nM PMA for 72 hours (THP-1 macrophages)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 07 2012

Thank you for contacting us.

This product has been tested by us in Flow Cytometry in RAW 264.7 cells. We have added this data to the datasheet. In regards to IHC-P, while this product is covered by our Abpromise guarantee in IHC-P in mouse...

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Gracias por tu respuesta.

Si en lugar de recibir ab27537 os es más conveniente recibir otro anticuerpo que haya sido testado en ratón mediante WB, no habría ningún problema.

De hecho yo recomendarí...

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1-10 of 19 Abreviews or Q&A