Overview

  • Product nameAnti-CD11b antibody [ICRF44]
    See all CD11b primary antibodies
  • Description
    Mouse monoclonal [ICRF44] to CD11b
  • SpecificityWe have data to indicate that this antibody may not cross react with Cat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Monkey, Baboon, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Rheumatoid synovial cells and human monocytes.

  • Positive control
    • Human tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab34216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
  • Application notesFlow Cyt: 1/10 - 1/100. Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.
    IHC-Fr: 1/100 - 1/1000.
    IP: Use at an assay dependent dilution.

    Is unsuitable for IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • Tissue specificityPredominantly expressed in monocytes and granulocytes.
    • Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    • Sequence similaritiesBelongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Anti-CD11b antibody [ICRF44] images

    • Ab34216, at a dilution of 1/10 - 1/100 (and then using 10µl of the suggested working dilution to label 106 cells or 100µl whole blood), staining CD11b in human peripheral blood granulocytes by Flow Cytometry.

    References for Anti-CD11b antibody [ICRF44] (ab34216)

    This product has been referenced in:
    • Zenkel M  et al. Proinflammatory cytokines are involved in the initiation of the abnormal matrix process in pseudoexfoliation syndrome/glaucoma. Am J Pathol 176:2868-79 (2010). IHC-Fr ; Human . Read more (PubMed: 20395431) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (eye, anterior segment)
    Specification eye, anterior segment
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Dr. Matthias Zenkel

    Verified customer

    Submitted Aug 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"