Anti-CD11b antibody [M1/70] (ab8878)

  Flow Cytometry - CD11b antibody (ab8878)

Ab8878 on human macrophages isolated from whole blood. (red - result with 95% expression, yellow - negative control)

  Immunohistochemistry (Frozen sections) - CD11b antibody (ab8878)

ab8878 staining CD11b in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% BSA for 1 hour at 250C was performed. The sample was incubated with primary antibody (1/100) for 12 hours at 40C in PBS. A Cy3®-conjugated donkey polyclonal to rat IgG was used at dilution at 1/500 as secondary antibody. Red staining in the image represents Cy3-CD11b, green - FITC-BrdU and blue is DAPI staining.

This image is a courtesy of Anonymous Abreview

See Abreview

  Immunohistochemistry (PFA perfusion fixed frozen sections) - CD11b antibody [M1/70] (ab8878)

ab8878 staining CD11b in Mouse brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). The sections were perfusion fixed with paraformaldehyde prior to blocking with TNB blocking buffer for 1 hour at 24°C. The primary antibody was diluted 1/100 in TNB blocking buffer and incubated with the sample for 24 hours at 4°C. An HRP Polymer-conjugated Goat anti-Rat polyclonal was used undiluted as the secondary antibody. Detected using a Streptavidin-Alexa 546 conjugate.

This image is courtesy of an Anonymous Abreview.

See Abreview