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Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.
Our Abpromise guarantee covers the use of ab25533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.3µg for 106 cells.|
|Blocking||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD11b with ab25533 at 0.1 μg/106 cells dilution. A Rat Anti-Mouse Ly-6G/Ly-6C-PE was used as the secondary antibody.