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C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes (Mouse)
Our Abpromise guarantee covers the use of ab25175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.3µg for 106 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|Blocking||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Flow cytometric analysis of BALB/c mouse splenocytes labelling CD11b with ab25175 at 0.1 μg/106 cells and labelling Ly-6G/Ly-6C with a Rat Anti-Mouse LY6g-FITC antibody (ab25024).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"