Anti-CD11b antibody [M1/70] (Phycoerythrin) (ab25175)

Overview

  • Product name
    Anti-CD11b antibody [M1/70] (Phycoerythrin)
    See all CD11b primary antibodies
  • Description
    Rat monoclonal [M1/70] to CD11b (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cyt, Blocking, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes (Mouse)

  • Positive control
    • Flow Cyt: Mouse bone marrow and spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Blocking Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease
    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Anti-CD11b antibody [M1/70] (Phycoerythrin) images

  • C57Bl/6 bone marrow cells stained with 0.125µg ab25175 (solid line) or 0.125µg rat IgG2b PE isotype control (dashed line).

References for Anti-CD11b antibody [M1/70] (Phycoerythrin) (ab25175)

This product has been referenced in:
  • Flotte TJ  et al. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol 111:112-24 (1983). Read more (PubMed: 6340516) »

See 1 Publication for this product

Product Wall

Product Anti-CD11b antibody [M1/70] (Phycoerythrin) (ab25175) is a rat monoclonal Ab, isotype IgG2b.

A suitable isotype control for this Ab is product Rat IgG2b, kappa Monoclonal [KLH/G2b-1-2] (R-PE) - Isotype Control (ab136585). The product ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up