Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)

Overview

  • Product name
    Anti-CD11b antibody [MEM-174] (Phycoerythrin)
    See all CD11b primary antibodies
  • Description
    Mouse monoclonal [MEM-174] to CD11b (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human granulocytes

  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Applications

Our Abpromise guarantee covers the use of ab28101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

Use 20µl for 106 cells or 100µl of whole blood.

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease
    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Images

  • Flow cytometry analysis of human peripheral blood cells labelling CD11b with ab28101.

  • ab28101 staining CD11b in Human peripheral blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/6) for 30 minutes at 4°C. Gating Strategy: Granulocytes.

    See Abreview

References

ab28101 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Human Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Cow Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Sheep Cell (Peripheral blood)
Specification
Peripheral blood
Gating Strategy
Granulocytes
Preparation
Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
Sample buffer: None
Username

Ms. Ina Laura Pieper

Verified customer

Submitted Sep 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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