Overview

  • Product name
    Anti-CD11b/c antibody [OX42]
    See all CD11b/c primary antibodies
  • Description
    Mouse monoclonal [OX42] to CD11b/c
  • Specificity
    Recognizes a common epitope shared by CD11b (ITGAM) and CD11c (ITGAX). It reacts with all macrophages in the peritoneal cavity, activated macrophages and Kupffer cells, but with only ~35% of alveolar macrophages. It also binds to all granulocytes and to dendritic cells, brain microglia, sparse thymic cortical cells and epidermal Langerhans cells. The antibody inhibits activity of the complement receptor type 3 (CR3) (PMID: 3512425).
  • Tested applications
    Suitable for: IHC-FoFr, ELISA, Flow Cyt, IHC-Fr, IHC-FrFl, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Rat
    Does not react with: Mouse
  • Immunogen

    Rat macrophages

  • Positive control
    • ICC/IF: NR8383 cells, rat macrophages. IHC-Frozen: rat spleen and brain.
  • General notes

    IHC protocol advice:

    This antibody is not suitable for IHC on paraffin-embedded samples.

    For best results in IHC on frozen tissue, the following may help detection:
    1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
    2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
    3) PFA-fixed samples will require cryoprotection by sucrose infiltration.

    4) Use OCT (TissueTek) compound for embedding.
    5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.

    6) Do not heat the samples or sections.

    7) During the staining procedure, do not allow the sections to dry out.

    Our Technical team (technical@abcam.com) will be happy to provide further information and advice.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b/c equivalent antigen.
  • Clonality
    Monoclonal
  • Clone number
    OX42
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
ELISA 1/100 - 1/3200.
Flow Cyt 1/25 - 1/200.

(10 µl per million cells).
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/500.
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 - 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 3512425
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • Tissue specificity
      Predominantly expressed in monocytes and granulocytes.
    • Involvement in disease
      Systemic lupus erythematosus 6
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • Domain
      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen CD11b (p170) antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11C antibody
      • Cell surface glycoprotein MAC 1 subunit alpha antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3A antibody
      • Integrin alpha M antibody
      • Integrin alpha X antibody
      • Integrin alpha-M antibody
      • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • ITGAX antibody
      • Leu M5, alpha subunit antibody
      • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Leukocyte adhesion receptor p150,95 antibody
      • Leukocyte surface antigen p150,95, alpha subunit antibody
      • MAC 1 antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • MO1A antibody
      • Myeloid membrane antigen, alpha subunit antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • OTTHUMP00000163299 antibody
      • p150 95 integrin alpha chain antibody
      • SLEB6 antibody
      see all

    Images

    • ab1211 staining CD11b/c (shown in red) in rat spleen tissue sections by IHC (PFA perfusion fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with BSA for 10 minutes at 21°C. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 16 hours. A Biotin-conjugated Goat anti mouse polyclonal (1/300) was used as the secondary antibody.

    • NR8383 cells (rat macrophage cell line) stained for CD11b/c (shown in green) using ab1211 in ICC/IF. The cells were fixed with 100% methanol for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% Tween-PBS for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (shown in blue) at a concentration of 1.43 µM for 1 hour at room temperature.

    • ab1211 staining CD11b/c (shown in green) in rat spleen tissue by IHC (Frozen sections). Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.

      See Abreview

    • ab1211 staining CD11b/c (shown in red) in rat brain tissue sections by IHC (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with NGS in 0.3% TritonX-PBS for 60 minutes at 24°C. The sample was incubated with primary antibody (1/300 in NGS in 0.3% TritonX-PBS) at 4°C for 42 hours. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150111) was used as the secondary antibody at 1/500.

      See Abreview

    References

    This product has been referenced in:
    • Sun Y  et al. Therapeutic effect of apocynin through antioxidant activity and suppression of apoptosis and inflammation after spinal cord injury. Exp Ther Med 13:952-960 (2017). IHC ; Rat . Read more (PubMed: 28450925) »
    • Yang H  et al. Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model. Front Pharmacol 8:44 (2017). IF . Read more (PubMed: 28239352) »

    See all 44 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spleen)
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 21 2016

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Brandon Jun

    Verified customer

    Submitted Dec 17 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lung)
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted May 31 2013

    According to current literature, both CD11b and CD11c are suitable markers for microglia (neuronal macrophages).

    Please see below for a reference:
    http://www.jneuroinflammation.com/content/4/1/26

    However, please note that...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (liver)
    Specification
    liver
    Username

    Abcam user community

    Verified customer

    Submitted Dec 23 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Tissue sections (Microglia - cell culture)
    Permeabilization
    No
    Specification
    Microglia - cell culture
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 25 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (adult liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Specification
    adult liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 15 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Rat Cell (Mixed primary glial cell culture)
    Specification
    Mixed primary glial cell culture
    Permeabilization
    No
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Mar 16 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Rat Cell (Primary Mixed Cortical Cultures)
    Specification
    Primary Mixed Cortical Cultures
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Ms. Nancy Nutile-McMenemy

    Verified customer

    Submitted Nov 09 2005

    1-10 of 12 Abreviews or Q&A

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