Anti-CD11b/c antibody [OX42] (FITC) (ab112170)

Overview

  • Product nameAnti-CD11b/c antibody [OX42] (FITC)
    See all CD11b/c primary antibodies
  • Description
    Mouse Monoclonal [OX42] to CD11b/c (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat Peritoneal Macrophages.

  • Positive control
    • Fisher Rat Peritoneal Macrophages.

Properties

Applications

Our Abpromise guarantee covers the use of ab112170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.01-0.1µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
    • Tissue specificityPredominantly expressed in monocytes and granulocytes.
    • Involvement in diseaseSystemic lupus erythematosus 6
    • Sequence similaritiesBelongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen CD11b (p170) antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11C antibody
      • Cell surface glycoprotein MAC 1 subunit alpha antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3A antibody
      • Integrin alpha M antibody
      • Integrin alpha X antibody
      • Integrin alpha-M antibody
      • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • ITGAX antibody
      • Leu M5, alpha subunit antibody
      • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Leukocyte adhesion receptor p150,95 antibody
      • Leukocyte surface antigen p150,95, alpha subunit antibody
      • MAC 1 antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • MO1A antibody
      • Myeloid membrane antigen, alpha subunit antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • OTTHUMP00000163299 antibody
      • p150 95 integrin alpha chain antibody
      • SLEB6 antibody
      see all

    Anti-CD11b/c antibody [OX42] (FITC) images

    • ab112170 at 0.06 µg/106 cells staining CD11b/c in Rat Peritoneal Macrophages (Fisher strain) by Flow Cytometry.
      Percentage of cells stained above control (FITC Mouse IgG2a, k): 99%.

    References for Anti-CD11b/c antibody [OX42] (FITC) (ab112170)

    ab112170 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Oct 03 2012

    Thank you for your enquiry and your interest in our products.

    I can confirm that this antibody (ab52478: Anti-CD11b antibody [EP1345Y]) is raised in rabbit; it has been tested for cross-reactivity only with mouse and human CD11b. It has not b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"