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Rheumatoid synovial fluid cells and fibronectin purified human monocytes.
Our Abpromise guarantee covers the use of ab11029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
Human peripheral blood lymphocytes stained with ab11029. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were stained with anti-CD11c ab11029 (right panel) at 1/100 dilution for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (left panel) was mouse monoclonal IgG1 (ab170190) used under the same conditions.
Acquisition of >30,000 total events were collected using a 50mW Argon Blue laser (488nm) and 530/30 bandpass filter. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable cells.
ab11029 staining CD11c in Mouse placenta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% BSA) for 90 minutes. An undiluted HRP-conjugated anti-mouse polyclonal was used as the secondary antibody.