Overview

  • Product nameAnti-CD11c antibody [3.9]
    See all CD11c primary antibodies
  • Description
    Mouse monoclonal [3.9] to CD11c
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Rheumatoid synovial fluid cells and fibronectin purified human monocytes.

  • Positive control
    • Flow Cyt: Human Leucocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab11029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt 1/100.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Anti-CD11c antibody [3.9] images

  • Human peripheral blood lymphocytes stained with ab11029. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were stained with anti-CD11c ab11029 (right panel) at 1/100 dilution for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (left panel) was mouse monoclonal IgG1 (ab170190) used under the same conditions.

    Acquisition of >30,000 total events were collected using a 50mW Argon Blue laser (488nm) and 530/30 bandpass filter. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable cells.

  • ab11029 staining CD11c in Mouse placenta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% BSA) for 90 minutes. An undiluted HRP-conjugated  anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • ab11029 staining CD11c in murine placenta and uterine tissue sections by Immunohistochemistry (Frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 25°C. Samples were incubated with primary antibody (1/25 in 1% BSA in PBS) for 1 hour at 25°C. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used to detect staining.

    See Abreview

References for Anti-CD11c antibody [3.9] (ab11029)

This product has been referenced in:
  • Le DD  et al. Allergic airway inflammation induces the migration of dendritic cells into airway sensory ganglia. Respir Res 15:73 (2014). ICC/IF ; Mouse . Read more (PubMed: 24980659) »
  • Katara GK  et al. Tumor-associated vacuolar ATPase subunit promotes tumorigenic characteristics in macrophages. Oncogene N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 24362525) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (oral mucosa (tongue))
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization No
Specification oral mucosa (tongue)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Prof. Luciana Correa

Verified customer

Submitted Jun 18 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Mouse Tissue sections (Breast tumor)
Specification Breast tumor
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Gajendra Katara

Verified customer

Submitted Sep 05 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Cell (Peritoneal macrophages)
Specification Peritoneal macrophages
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 21 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step casein in PBS- DAKO ready to use sera-free protein block as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Traci Lyons

Verified customer

Submitted Nov 13 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample Mouse Tissue sections (Uterine Decidua)
Specification Uterine Decidua
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Nov 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Placenta)
Specification Placenta
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3µg/mL · Temperature: RT°C
Username

Dr. Mukesh Jaiswal

Verified customer

Submitted Sep 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Placenta and uterus)
Specification Placenta and uterus
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Varkha Agrawal

Verified customer

Submitted Sep 18 2012

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