The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Staining of normal Human peripheral blood cells with Mouse IgG1 ? Isotype Control APC (open histogram) or ab95764 (filled histogram). Cells in the monocyte gate were used for analysis.
Flow Cytometry - Anti-CD11c antibody [3.9] (Allophycocyanin) (ab95764)This image is courtesy of an anonymous Abreview
ab36956 staining CD11c in the Human PBMCs by Flow Cytometry. Cells were harvested in a Ficoll-hypaque gradient. The sample was incubated with the primary antibody (1/100 in 2% Human sserum, 1mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes