Overview

  • Product nameAnti-CD11c antibody [8A2]See all CD11c primary antibodies ...
  • Description
    Mouse monoclonal [8A2] to CD11c
  • Specificityab53441 recognises the rat CD11c cell surface antigen, expressed by some myeloid cells and dendritic cells. The antibody immunoprecipitates a 130-140/90kD heterodimer and modestly inhibits macrophage adherence on plastic (by around 20-30%).
  • Tested applicationsIP, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation; Rat alveolar macrophages

Properties

Applications

Our Abpromise guarantee covers the use of ab53441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent dilution.
IHC-Fr Use a concentration of 10 µg/ml.
Flow Cyt 1/10 - 1/50.

Target

  • FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P20702 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

References for Anti-CD11c antibody [8A2] (ab53441)

This product has been referenced in:
  • Manley NC  et al. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat. PLoS One 8:e61789 (2013). Flow Cyt ; Rat . Read more (PubMed: 23613937) »
  • Quan LD  et al. Development of a macromolecular prodrug for the treatment of inflammatory arthritis: mechanisms involved in arthrotropism and sustained therapeutic efficacy. Arthritis Res Ther 12:R170 (2010). Flow Cyt ; Rat . Read more (PubMed: 20836843) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Spleen)
Specification Rat Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Permeabilization Yes - .01% Triton-X-100
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: 22°C
Username

Payal Mital

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"