Anti-CD11c antibody [BU15], prediluted (PerCP) (ab74685)

Overview

  • Product nameAnti-CD11c antibody [BU15], prediluted (PerCP)
    See all CD11c primary antibodies
  • Description
    Mouse monoclonal [BU15] to CD11c, prediluted (PerCP)
  • ConjugationPerCP. Ex: 482nm, Em: 675nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Dendritic cells of synovial fluid

  • Positive control
    • Human blood cells
  • General notes

    Purified antibody is conjugated with Peridinin chlorophyll protein complex (PerCP) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab74685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Anti-CD11c antibody [BU15], prediluted (PerCP) images

  • ab74685 staining surface CD11c in human peripheral blood cells by Flow Cytometery. Monocytes were used for gating. The blue peak repersents negative control while red peak shows staining with ab74685. 

References for Anti-CD11c antibody [BU15], prediluted (PerCP) (ab74685)

This product has been referenced in:
  • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . Read more (PubMed: 24388011) »
  • Salimi M  et al. A role for IL-25 and IL-33-driven type-2 innate lymphoid cells in atopic dermatitis. J Exp Med 210:2939-50 (2013). Flow Cyt ; Human . Read more (PubMed: 24323357) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
Sample buffer: PBS
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

Submitted May 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"