Recombinant
RabMAb

Anti-CD11c antibody [EP1347Y] (ab52632)

Overview

  • Product name
    Anti-CD11c antibody [EP1347Y]
    See all CD11c primary antibodies
  • Description
    Rabbit monoclonal [EP1347Y] to CD11c
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD11c aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P20702

  • Positive control
    • THP-1 + TPA lysate. Human lymph node. Flow: Human peripheral blood lymphocytes.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
IP 1/20.

For unpurified use at 1/80.

Target

  • Function
    Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Images

  • Unpurified ab52632 showing positive staining in Normal tonsil tissue.

  • Anti-CD11c antibody [EP1347Y] (ab52632) at 1/1000 dilution (unpurified) + THP-1 + TPA lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 128 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD11c antibody [EP1347Y] (ab52632) at 1/1000 dilution (unpurified)

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52632 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Unpurified ab52632 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with ab52632 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Unpurified ab52632 showing negative staining in Normal brain tissue.

  • Unpurified ab52632 showing negative staining in Normal breast tissue.

  • Unpurified ab52632 showing negative staining in Skeletal muscle tissue.

  • Unpurified ab52632 showing positive staining in Normal spleen tissue.

References

This product has been referenced in:
  • Furukawa S  et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep 7:42413 (2017). IHC ; Human . Read more (PubMed: 28205524) »
  • Zhang X  et al. Protective effect of curcumin on TNBS-induced intestinal inflammation is mediated through the JAK/STAT pathway. BMC Complement Altern Med 16:299 (2016). IHC-P ; Rat . Read more (PubMed: 27544348) »

See all 20 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Permeabilization
No
Specification
Skin
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Human Sarcoma
Blocking step
Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Rabbit Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
Yes - 0.2% Tween in PBS
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 25 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6.0
Sample
Cynomolgus Monkey Tissue sections (Tonsil, lymph node, spleen, thymus)
Specification
Tonsil, lymph node, spleen, thymus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: Rt°C
Fixative
Formaldehyde
Username

Jing Ma

Verified customer

Submitted Dec 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 16.7% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.1
Sample
Human Tissue sections (colon)
Specification
colon
Permeabilization
No
Fixative
Formaldehyde
Username

Ms. Judith Gras

Verified customer

Submitted Sep 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 16.7% · Temperature: 22°C
Sample
Human Tissue sections (colon)
Specification
colon
Permeabilization
Yes - Acetone
Fixative
Acetone
Username

Ms. Judith Gras

Verified customer

Submitted Sep 24 2013


Yes, if the datasheet says does not react with mouse , it means this antibody will not worl on mouse samples.
We do have mouse specific antibodies for Anti-CD11c raised in hamster which works really well.
A suggested antibody that is wid...

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Kein Problem - wir haben soeben den Discount Code hinzugefügt. Ich kann Ihnen bestätigen, dass dieser jetzt aktiv ist.

Es freut mich auch zu sehen, dass der Antikörper auf gefroren Schnitten funktioniert!

Bitte zö...

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1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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