Anti-CD11c antibody [N418] (ab33483)
Key features and details
- Armenian hamster monoclonal [N418] to CD11c
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CD11c antibody [N418]
See all CD11c primary antibodies -
Description
Armenian hamster monoclonal [N418] to CD11c -
Host species
Armenian hamster -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
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General notes
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo. -
Clonality
Monoclonal -
Clone number
N418 -
Myeloma
Sp2/0 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD11c antibody [N418] (ab111469)
- Anti-CD11c antibody [N418] - Low endotoxin, Azide free (ab185783)
- APC Anti-CD11c antibody [N418] (ab210306)
- APC/Cy7® Anti-CD11c antibody [N418] (ab210307)
- FITC Anti-CD11c antibody [N418] (ab210308)
- PE Anti-CD11c antibody [N418] (ab210309)
- PE/Cy7® Anti-CD11c antibody [N418] (ab210310)
- Alexa Fluor® 647 Anti-CD11c antibody [N418] (ab233046)
- Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
- violetFluor™ 450 Anti-CD11c antibody [N418] (ab242269)
- Alexa Fluor® 488 Anti-CD11c antibody [N418] (ab33503)
- Biotin Anti-CD11c antibody [N418] (ab95765)
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
1/100.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
1/100. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16411 Mouse
- SwissProt: Q9QXH4 Mouse
- Unigene: 22378 Mouse
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
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Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes. -
Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (77)
ab33483 has been referenced in 77 publications.
- Zhao L et al. Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ1 signaling pathway in diabetic atherosclerosis. Mol Med 28:4 (2022). PubMed: 35062863
- De Marco M et al. Concerted BAG3 and SIRPα blockade impairs pancreatic tumor growth. Cell Death Discov 8:94 (2022). PubMed: 35241649
- Zhang J et al. Characterization of the Immunologic Phenotype of Dendritic Cells Infected With Herpes Simplex Virus 1. Front Immunol 13:931740 (2022). PubMed: 35865514
- Han SK et al. Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation. BMC Microbiol 21:146 (2021). PubMed: 33985438
- Lin Z et al. Uptake and trafficking of different sized PLGA nanoparticles by dendritic cells in imiquimod-induced psoriasis-like mice model. Acta Pharm Sin B 11:1047-1055 (2021). PubMed: 33996416