• Product nameAnti-CD11c antibody [N418]
    See all CD11c primary antibodies
  • Description
    Armenian Hamster monoclonal [N418] to CD11c
  • Specificityab33483 recognizes the murine homologue of human CD11c (150/90kD) integrin family. This product is recommended for IHC-Fr only although some customers have used it successfully in IHC-P.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Spleen dendritic cells (Mouse)

  • Positive control
    • IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
  • General notes

    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.



Our Abpromise guarantee covers the use of ab33483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20808846
Flow Cyt 1/100.

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Abcam recommends using acetone fixation.

IP Use at an assay dependent concentration.


  • FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificityPredominantly expressed in monocytes and granulocytes.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Anti-CD11c antibody [N418] images

  • Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488)  preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

    Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

  • Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.

    See Abreview

  • ab33483 staining CD11c in murine bronchoalveolar lavage (BAL) cells by Immunocytochemistry/ Immunofluorescence.
    BAL cells were collected from PBS treated mice at day 13, 17 or 18 (17/18) and 21 post-reconstitution. Cells were stained with ab33483 specific for CD11c (green) and Pc (red). Representative images of brightfield (BF), CD11c, Pc, and merged CD11c/Pc are shown for PBS-treated mice following immune reconstitution Day 21.

References for Anti-CD11c antibody [N418] (ab33483)

This product has been referenced in:
  • Zhang J  et al. Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice. PLoS Pathog 11:e1005001 (2015). IHC ; Human . Read more (PubMed: 26107716) »
  • Nurnberg ST  et al. Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap. PLoS Genet 11:e1005155 (2015). Mouse . Read more (PubMed: 26020946) »

See all 24 Publications for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (Cells from bronchoaveolar lavage in balb/c mice)
Permeabilization No
Gating Strategy CD45+, C11b+, GR1-cells
Specification Cells from bronchoaveolar lavage in balb/c mice
Preparation Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation None, cells were used fresh

Dr. Francois Daubeuf

Verified customer

Submitted Sep 29 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step None
Permeabilization No
Specification Lung
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative Zamboni

Mr. Duc Dung Le

Verified customer

Submitted Apr 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Mar 28 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 08 2014

The appropriate secondary antibody for use with this product will be a Secondary Antibody to Armenian Hamster IgG such as ab5744.

Thank you for your reply.

I would like to recommend the following:

Primary antibody: ab6640 (F4/80 antibody)
Secondary antibody: ab99671 (Mouse monoclonal to Rat IgG2b - FITC)

compatible with


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In order to assist you further, I would like to clarify the details of your experiment. You mention that you are going to be performing IHC - will this be on frozen or ...

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Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would...

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Thank you for your enquiry and for this further information.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, ...

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