Overview

  • Product name
    Anti-CD11c antibody [N418]
    See all CD11c primary antibodies
  • Description
    Armenian Hamster monoclonal [N418] to CD11c
  • Specificity
    ab33483 recognizes the murine homologue of human CD11c (150/90kD) integrin family. This product is recommended for IHC-Fr only although some customers have used it successfully in IHC-P.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD11c.

  • Positive control
    • IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
  • General notes


    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Properties

Applications

Our Abpromise guarantee covers the use of ab33483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20808846
Flow Cyt 1/100.

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Abcam recommends using acetone fixation.

IP Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

Images

  • Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488)  preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.


    Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

  • Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.

    See Abreview

  • ab33483 staining CD11c in murine bronchoalveolar lavage (BAL) cells by Immunocytochemistry/ Immunofluorescence.
    BAL cells were collected from PBS treated mice at day 13, 17 or 18 (17/18) and 21 post-reconstitution. Cells were stained with ab33483 specific for CD11c (green) and Pc (red). Representative images of brightfield (BF), CD11c, Pc, and merged CD11c/Pc are shown for PBS-treated mice following immune reconstitution Day 21.

References

This product has been referenced in:
  • Tan CSE  et al. CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection. PLoS Pathog 13:e1006311 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28394921) »
  • Xu J  et al. The REG?-proteasome forms a regulatory circuit with I?B? and NF?B in experimental colitis. Nat Commun 7:10761 (2016). IHC-P ; Mouse . Read more (PubMed: 26899380) »

See all 33 Publications for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain Cortex in male and female mice)
Specification
Brain Cortex in male and female mice
Username

Helena Morrison

Verified customer

Submitted Sep 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, simmering boil, 20 min
Permeabilization
Yes - deparaffinization
Specification
adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraffin-imbedded
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Application
Flow Cytometry
Sample
Mouse Cell (Cells from bronchoaveolar lavage in balb/c mice)
Permeabilization
No
Gating Strategy
CD45+, C11b+, GR1-cells
Specification
Cells from bronchoaveolar lavage in balb/c mice
Preparation
Cell harvesting/tissue preparation method: Bronchoaveolar lavage in balb/c mice was performed with PBS-EDTA 3mM
Sample buffer: PBS-EDTA 3mM
Fixation
None, cells were used fresh
Username

Dr. Francois Daubeuf

Verified customer

Submitted Sep 29 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative
Zamboni
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Mouse Tissue sections (bone)
Specification
bone
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 28 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 08 2014

The appropriate secondary antibody for use with this product will be a Secondary Antibody to Armenian Hamster IgG such as ab5744.

Thank you for your reply.


I would like to recommend the following:


Primary antibody: ab6640 (F4/80 antibody)
Secondary antibody: ab99671 (Mouse monoclonal to Rat IgG2b - FITC)


compatible with


...

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Thank you for contacting Abcam and for your interest in our products.


In order to assist you further, I would like to clarify the details of your experiment. You mention that you are going to be performing IHC - will this be on frozen or ...

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Thank you for contacting us. Your credit note ID is xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice...

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1-10 of 24 Abreviews or Q&A

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