The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells. Final volume of 100 µl.
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
C57Bl/6 splenocytes were stained with APC-conjugated anti-MHC CLass II and 0.03µg ab95765 (right) or 0.03µg biotin-conjugated armenian hamster IgG isotype control (left), followed by streptavidin PE.
References for Anti-CD11c antibody [N418] (Biotin) (ab95765)
This product has been referenced in:
Xin KQ et al. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol80:11899-910 (2006).
Read more (PubMed: 17005662) »
Ohteki T et al. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med203:2329-38 (2006).
Read more (PubMed: 16966429) »