Anti-CD13 antibody [CVS19] (ab23758)

Overview

  • Product nameAnti-CD13 antibody [CVS19]
    See all CD13 primary antibodies
  • Description
    Mouse monoclonal [CVS19] to CD13
  • SpecificityThis antibody recognises the CD13 cell surface antigen expressed by granulocytes and monocytes.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Horse
  • Immunogen

    Leucocytes. (Horse)

Properties

Applications

Our Abpromise guarantee covers the use of ab23758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/200 - 1/500. Predicted molecular weight: 109 kDa.

Target

  • FunctionBroad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • Tissue specificityExpressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • Sequence similaritiesBelongs to the peptidase M1 family.
  • DomainAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • Post-translational
    modifications
    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • Cellular localizationCell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt
  • Alternative names
    • Alanyl (membrane) aminopeptidase antibody
    • Alanyl aminopeptidase antibody
    • Aminopeptidase M antibody
    • Aminopeptidase N antibody
    • AMPN_HUMAN antibody
    • ANPEP antibody
    • AP M antibody
    • AP N antibody
    • AP-M antibody
    • AP-N antibody
    • APN antibody
    • CD 13 antibody
    • CD13 antibody
    • CD13 antigen antibody
    • gp150 antibody
    • hAPN antibody
    • LAP 1 antibody
    • LAP1 antibody
    • Microsomal aminopeptidase antibody
    • Myeloid plasma membrane glycoprotein CD13 antibody
    • p150 antibody
    • PEPN antibody
    see all

Anti-CD13 antibody [CVS19] images

  • All lanes : Anti-CD13 antibody [CVS19] (ab23758) at 1/200 dilution

    Lane 1 : Horse seminal plasma
    Lane 2 : Horse leucocyte lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 109 kDa
    Observed band size : 100,110 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

    This image is courtesy of an Abreview submitted by Guillaume Tsikis

    See Abreview

References for Anti-CD13 antibody [CVS19] (ab23758)

ab23758 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (8-16%)
Sample Horse Serum (seminal plasma)
Specification seminal plasma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Guillaume TSIKIS

Verified customer

Submitted Oct 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"