The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 110 kDa).
1/10 - 1/100.
1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt.
FunctionBroad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Tissue specificityExpressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Sequence similaritiesBelongs to the peptidase M1 family.
DomainAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
Post-translational modificationsSulfated. N- and O-glycosylated. May undergo proteolysis and give rise to a soluble form.
Cellular localizationCell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)This image is courtesy of an anonymous abreview.
ab108382 staining CD13 in human kidney tissue sections by Immunohistochemistry (Formaldehyde/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween-20 and blocked for 60 minutes at 25°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody at a dilution of 1/400 for 1 hour at 25°C. An Alexa Flour® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1200) was used as the secondary antibody.
Western blot - CD13 antibody [EPR4059] (ab108382)
All lanes : Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution
Lane 1 : THP-1 cell lysate Lane 2 : U937 cell lysate Lane 3 : A375 cell lysate