FITC Anti-CD13 antibody [WM15] (ab52461)
Key features and details
- FITC Mouse monoclonal [WM15] to CD13
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD13 antibody [WM15]
See all CD13 primary antibodies -
Description
FITC Mouse monoclonal [WM15] to CD13 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD13. Human AML cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Prior to conjugation the antibody was purified by protein A affinity chromatography. Following conjugation, it was purified by size exclusion chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
WM15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-CD13 antibody [WM15] (ab130430)
- PerCP/Cy5.5® Anti-CD13 antibody [WM15] (ab157316)
- Alexa Fluor® 488 Anti-CD13 antibody [WM15] (ab239271)
- APC Anti-CD13 antibody [WM15] (ab239295)
- PE/DyLight™ 594 Anti-CD13 antibody [WM15] (ab239330)
- Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
- PE Anti-CD13 antibody [WM15], prediluted (ab69775)
- Anti-CD13 antibody [WM15] (ab7417)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 20µl for 106 cells.
or 100 µl of whole blood. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 20µl for 106 cells. or 100 µl of whole blood. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
Tissue specificity
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
Sequence similarities
Belongs to the peptidase M1 family. -
Domain
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
Post-translational
modificationsSulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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Database links
- Entrez Gene: 290 Human
- Omim: 151530 Human
- SwissProt: P15144 Human
- Unigene: 1239 Human
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Alternative names
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab52461 has been referenced in 1 publication.
- Bork S et al. DNA methylation pattern changes upon long-term culture and aging of human mesenchymal stromal cells. Aging Cell 9:54-63 (2010). PubMed: 19895632