Anti-CD130 (gp130) antibody [B-R3] (FITC) (ab47218)

Overview

  • Product name
    Anti-CD130 (gp130) antibody [B-R3] (FITC)
    See all CD130 (gp130) primary antibodies
  • Description
    Mouse monoclonal [B-R3] to CD130 (gp130) (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognizes CD130 (gp130) (130-140 kDa); common subunit for IL6, IL11, OSM, LIF, CNTF and CT1 receptors.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human): natural soluble CD130 (gp130).

Properties

Applications

Our Abpromise guarantee covers the use of ab47218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl for 106 cells or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
    • Tissue specificity
      Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 5 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Domain
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      modifications
      Phosphorylation of Ser-782 down-regulates cell surface expression.
      Heavily N-glycosylated.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD130 antibody
      • CD130 antigen antibody
      • CDw130 antibody
      • gp130 antibody
      • GP130 RAPS antibody
      • IL 6R beta antibody
      • IL-6 receptor subunit beta antibody
      • IL-6R subunit beta antibody
      • IL-6R-beta antibody
      • IL-6RB antibody
      • IL6 ST antibody
      • IL6RB_HUMAN antibody
      • IL6ST antibody
      • Interleukin 6 receptor subunit beta antibody
      • Interleukin receptor beta chain antibody
      • Interleukin-6 receptor subunit beta antibody
      • Interleukin-6 signal transducer antibody
      • Membrane glycoprotein 130 antibody
      • Membrane glycoprotein gp130 antibody
      • Oncostatin M receptor antibody
      • Oncostatin M receptor alpha subunit antibody
      • Oncostatin-M receptor subunit alpha antibody
      see all

    Images

    • Ab47218, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD130 (gp130) by Flow Cytometry.

    References

    This product has been referenced in:
    • Redell MS  et al. FACS analysis of Stat3/5 signaling reveals sensitivity to G-CSF and IL-6 as a significant prognostic factor in pediatric AML: a Children's Oncology Group report. Blood 121:1083-93 (2013). Flow Cyt ; Human . Read more (PubMed: 23243289) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    The concentration of the lot you have is 34.7 ug/ml, or 347 ng per test (10 ul).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for your reply.




    This product would also be compatible and is simply a different clone of isotype control antibody. The target and conjugation remain the same.


    I hope this information is helpful. Please do...

    Read More

    Thank you for contacting Abcam regarding ab47218.


    Regarding an isotype control, I recommend ab91362 to use with this primary antibody.


    http://www.abcam.com/Mouse-IgG2a-X5563-FITC-Isotype-Control-ab91362.html


    I ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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