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Our Abpromise guarantee covers the use of ab16518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.
Fix with 3% paraformaldehyde for 20 min at RT.
|Flow Cyt||1/10 - 1/50.|
|IHC-P||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).|
|ELISA||Use at an assay dependent concentration.|
|IHC-FoFr||1/100. PubMed: 17320377|
Paraformaldehyde-fixed mouse mammary carcinoma tissue stained for CD133 (red) using ab16518 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of CEM cells using ab16518 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"