Recombinant Anti-CD134 / OX40L receptor antibody [EPR17Y] - BSA and Azide free (ab167545)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD134 / OX40L receptor antibody [EPR17Y] - BSA and Azide free
See all CD134 / OX40L receptor primary antibodies -
Description
Rabbit monoclonal [EPR17Y] to CD134 / OX40L receptor - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD134/ OX40L receptor (extracellular). The exact sequence is proprietary.
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Positive control
- PMBC cell lysates treated with TPA.
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General notes
ab167545 is the carrier-free version of ab76000.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa).
Detects a band of approximately 45 kDa due to glycoslyation (predicted molecular weight: 30 kDa). |
Notes |
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Flow Cyt
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa). Detects a band of approximately 45 kDa due to glycoslyation (predicted molecular weight: 30 kDa). |
Target
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Function
Receptor for TNFSF4/OX40L/GP34. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7293 Human
- Omim: 600315 Human
- SwissProt: P43489 Human
- Unigene: 129780 Human
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Alternative names
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
Images
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All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/5000 dilution
Lane 1 : Human lymph node tissue lysate
Lane 2 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab76000, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab76000, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CD134 / OX40L receptor antibody [EPR17Y] (ab76000) at 1/500 dilution (un-purified)
Lane 1 : PBMC cell lysates, untreated
Lane 2 : PBMC cell lysates, treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using ab76000, the same antibody clone in a different buffer formulation.
Note: the actual molecular weight is larger than the predicted due to glycoslyation.
Datasheets and documents
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Datasheet download
References (0)
ab167545 has not yet been referenced specifically in any publications.