Overview

  • Product nameAnti-CD134 / OX40 antibody [EPR17Y]
    See all CD134 / OX40 primary antibodies
  • Description
    Rabbit monoclonal [EPR17Y] to CD134 / OX40
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD134/ OX40 aa 1-250. The exact sequence is proprietary.

  • Positive control
    • WB- Human lymph node, Human spleen, Human tonsil and PMBC cell lysates treated with TPA.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 23897980
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 30 kDa).
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionReceptor for TNFSF4/OX40L/GP34.
    • Sequence similaritiesContains 4 TNFR-Cys repeats.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACT 35 antibody
      • ACT35 antibody
      • ACT35 antigen antibody
      • ATC35 antigen antibody
      • CD 134 antibody
      • CD134 antibody
      • CD134 antigen antibody
      • IMD16 antibody
      • Lymphoid activation antigene ACT35 antibody
      • OX 40 antibody
      • OX40 antibody
      • OX40 antigen antibody
      • OX40 cell surface antigen antibody
      • OX40 homologue antibody
      • OX40L receptor antibody
      • TAX transcriptionally activated glycoprotein 1 receptor antibody
      • TAX transcriptionally-activated glycoprotein 1 receptor antibody
      • TNF receptor superfamily member 4 antibody
      • TNFRSF 4 antibody
      • TNFRSF4 antibody
      • TNR4_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 4 antibody
      • Txgp 1l antibody
      • Txgp1 antibody
      • Txgp1l antibody
      see all

    Anti-CD134 / OX40 antibody [EPR17Y] images

    • All lanes : Anti-CD134 / OX40 antibody [EPR17Y] (ab76000) at 1/5000 dilution

      Lane 1 : Human lymph node
      Lane 2 : Human spleen

      Lysates/proteins at 10 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Blocking and diluting buffer 5% NFDM/TBST
    • Anti-CD134 / OX40 antibody [EPR17Y] (ab76000) at 1/1000 dilution + Human tonsil at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Blocking and diluting buffer 5% NFDM/TBST
    • All lanes : Anti-CD134 / OX40 antibody [EPR17Y] (ab76000) at 1/500 dilution (un-purified)

      Lane 1 : PMBC cell lysates, untreated
      Lane 2 : PMBC cell lysates, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Note: the actual molecular weight is larger than the predicted due to glycoslyation.

    References for Anti-CD134 / OX40 antibody [EPR17Y] (ab76000)

    This product has been referenced in:
    • Byun M  et al. Inherited human OX40 deficiency underlying classic Kaposi sarcoma of childhood. J Exp Med 210:1743-59 (2013). WB, Flow Cyt ; Human . Read more (PubMed: 23897980) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab76000.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"