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Human peripheral blood mononuclear cells.
Though we have received a positive Abreview for this antibody in IHC-P, we have also received many negative reports in IHC-P and so we can no longer guarantee this antibody in this application. We appreciate any further feedback from users in this regard.
ab63319 reacts with peripheral blood monocytes and weakly with granulocytes. It is negative to platelets, erythrocytes and lymphocytes. It is sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). It is negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab133335 as a replacement.
Our Abpromise guarantee covers the use of ab63319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/25. Dilute with isotonic buffer. Use 50ul per 1x106 peripheral blood mononuclear cells (PBMC) in 100ul buffer.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/25. We recommend acetone fixation.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"