Human peripheral blood mononuclear cells.
ab63319 reacts with peripheral blood monocytes and weakly with granulocytes. It is negative to platelets, erythrocytes and lymphocytes. It is sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). It is negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).
Our Abpromise guarantee covers the use of ab63319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/25. Dilute with isotonic buffer. Use 50ul per 1x106 peripheral blood mononuclear cells (PBMC) in 100ul buffer.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/25. We recommend acetone fixation.|