Anti-CD14 antibody [47-3D6] (Allophycocyanin) (ab91144)

Overview

  • Product name
    Anti-CD14 antibody [47-3D6] (Allophycocyanin)
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [47-3D6] to CD14 (Allophycocyanin)
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity
    ab91144 reacts with Human monocytes and macrophages; weak reactions may occur with neutrophils.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human lung angiotensin converting enzyme.

  • Positive control
    • Human normal peripheral blood.

Properties

Applications

Our Abpromise guarantee covers the use of ab91144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • Tissue specificity
      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • Sequence similarities
      Contains 11 LRR (leucine-rich) repeats.
    • Post-translational
      modifications
      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Anti-CD14 antibody [47-3D6] (Allophycocyanin) images

    • ab91144, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.
    • ab91144, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.

    References for Anti-CD14 antibody [47-3D6] (Allophycocyanin) (ab91144)

    ab91144 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll-Hypaque density gradient
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    monocytes
    Username

    Abcam user community

    Verified customer

    Submitted Apr 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up