The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
ab91146, at 20µl/106 cells, staining CD14 in normal Human peripheral blood by Flow Cytometry.
References for Anti-CD14 antibody [47-3D6] (PerCP) (ab91146)
This product has been referenced in:
Hünniger K et al. A virtual infection model quantifies innate effector mechanisms and candida albicans immune escape in human blood. PLoS Comput Biol10:e1003479 (2014).
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