Overview

  • Product name
    Anti-CD14 antibody [61D3]
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [61D3] to CD14
  • Specificity
    Human CD14
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to CD14. Human peripheral monocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab25625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. PubMed: 21789185

Target

  • Function
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities
    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Images

  • Flow Cytometry analysis of Human monocyte cells labeling CD14 with ab25625 at 1 μg/106 cells (purple). A Goat Anti-Mouse IgG1, Human ads-CY5 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG1-UNLB, followed by Goat Anti-Mouse IgG1, Human ads-CY5.

  • Human peripheral blood cells stained with ab25625 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab25625, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Effect of CD14 neutralisation by ab25625 on LPS-induced cytokine release from alveolar macrophages, ATII and TT1 cells.

    Cells were incubated for 1 hr with 10 µg/ml ab25625 prior to addition of 100 ng/ml LPS for 24 hrs. The corresponding IgG isotypes were used as negative controls.

References

This product has been referenced in:
  • Thorley AJ  et al. Innate Immune Responses to Bacterial Ligands in the Peripheral Human Lung - Role of Alveolar Epithelial TLR Expression and Signalling. PLoS One 6:e21827 (2011). Neutralising ; Human . Read more (PubMed: 21789185) »
  • Reddy K  et al. Bone marrow subsets differentiate into endothelial cells and pericytes contributing to Ewing's tumor vessels. Mol Cancer Res 6:929-36 (2008). IHC-Fr ; Mouse . Read more (PubMed: 18567797) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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