The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab154457 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Contains 11 LRR (leucine-rich) repeats.
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG1 kappa Isotype Control PE-Cy7 (open histogram) or ab155360 (filled histogram). Cells in the monocyte gate were used for analysis.