Overview

  • Product name
  • Description
    Rabbit polyclonal to CD14
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 25-285 of Human CD14 (BC010507).

  • Positive control
    • Human fetal thymus and Hep2 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab106285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 40 kDa.

Target

  • Function
    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificity
    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similarities
    Contains 11 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Images

  • All lanes : Anti-CD14 antibody (ab106285) at 1/1000 dilution

    Lane 1 : Human fetal thymus lysate
    Lane 2 : Hep2 cell lysate


    Predicted band size : 40 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your telephone enquiry earlier this afternoon.

I can confirm that both these antibodies can be kept in the fridge at 4oC overnight until your colleagues return tomorrow.They will then need to store as directed:

For a...

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DISCOUNT CODE: ****** (no need to mention this code on the original order for ab106285)
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab106285 in Dog. This code will give you: 1 freepr...

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Thank you very much for your interest in ab106285.

To our knowledge,ab106285 has not been tested in Dog. The homology between the immunogen and the Dog CD14 is 70%, see attachment.

Because we carry over 70,000 products, it isn't fe...

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DISCOUNT CODE: 0000
Expiration date: 18. September 2012

I am very pleased to hear you would like to accept our offer and test ab106285 in mouse. This code will give you: the value of ab106285 off your next order before the expiration da...

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Thank you very much for your interest in ab106285.
I can confirm that ab106285 is tested and guaranteed for IHC-P.
To our knowledge, ab106285 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab1...

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DISCOUNT CODE: xxxxxxxx Expiration date: xxxxxxx I am very pleased to hear you would like to accept our offer and test ab106285 in Rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Irina Teplova

Verified customer

Submitted Jul 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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