The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells or 100 µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
A typical staining pattern of monocytes using ab27388.
References for Anti-CD14 antibody [B-A8] (ab27388)
This product has been referenced in:
Game DS et al. CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients. J Am Soc Nephrol14:1652-61 (2003).
Read more (PubMed: 12761268) »