Specificityab28118 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. ab28118 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Analysis by flow cytometry of ab28118 staining CD14 in human peripheral blood cells.
References for Anti-CD14 antibody [MEM-15] (Biotin) (ab28118)
This product has been referenced in:
Horejsí V et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha)32:12-25 (1986).
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