Anti-CD14 antibody [MEM-15] (FITC) (ab28061)


  • Product nameAnti-CD14 antibody [MEM-15] (FITC)
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [MEM-15] to CD14 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
  • Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

  • General notes

    The antibody is free of unconjugated FITC.



Our Abpromise guarantee covers the use of ab28061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells. or 100 µl of whole blood.

ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionCooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
  • Tissue specificityExpressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
  • Sequence similaritiesContains 11 LRR (leucine-rich) repeats.
  • Post-translational
    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 14 antibody
    • CD_antigen=CD14 antibody
    • CD14 antibody
    • CD14 antigen antibody
    • CD14 molecule antibody
    • CD14_HUMAN antibody
    • LPS-R antibody
    • Mo2 antibody
    • Monocyte differentiation antigen CD14 antibody
    • Monocyte differentiation antigen CD14 urinary form antibody
    • Monocyte differentiation antigen CD14, membrane-bound form antibody
    • Myeloid cell specific leucine rich glycoprotein antibody
    • Myeloid cell-specific leucine-rich glycoprotein antibody
    see all

Anti-CD14 antibody [MEM-15] (FITC) images

  • ab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.

    See Abreview

  • ab28061 staining CD14 in human peripheral blood monocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 2% paraformaldehyde for 20 minutes at room temperature and blocked with 2% goat serum for 15 minutes at 25°C. Samples were incubated with the undiluted primary antibody for 45 minutes at 25°C.

    See Abreview

References for Anti-CD14 antibody [MEM-15] (FITC) (ab28061)

This product has been referenced in:
  • Steensma MR  et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS One 8:e69101 (2013). Flow Cyt ; Human . Read more (PubMed: 23922683) »
  • Sing A  et al. Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med 196:1017-24 (2002). Read more (PubMed: 12391013) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (Peripheral Blood Monocyte)
Specification Peripheral Blood Monocyte
Permeabilization No
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 15 2014

Thank you for your inquiry. We do not recommend to freeze this antibody and to especially avoid freeze/thaw cycles. But I would like to recommend to run a test experiment. Antibodies in general are very stable and as long as the conjuga...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral Blood Mononuclear Cell)
Specification Peripheral Blood Mononuclear Cell
Fixation none
Permeabilization No
Gating Strategy monocytes

Abcam user community

Verified customer

Submitted Oct 20 2009