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Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
The antibody is free of unconjugated FITC.
Our Abpromise guarantee covers the use of ab28061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 20µl for 106 cells.
or 100 µl of whole blood.
ab28061 staining CD14 in human peripheral blood monocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 2% paraformaldehyde for 20 minutes at room temperature and blocked with 2% goat serum for 15 minutes at 25°C. Samples were incubated with the undiluted primary antibody for 45 minutes at 25°C.