Overview

  • Product name
    Anti-CD14 antibody [RPA-M1]
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [RPA-M1] to CD14
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Human monocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab86895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 0.1 - 0.2 µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • Tissue specificity
      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • Sequence similarities
      Contains 11 LRR (leucine-rich) repeats.
    • Post-translational
      modifications
      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Cell surface flow cytometric analysis of CD14 in human monocytes, using 0.1 µg of ab86895 for 106 cells. Anti mouse IgG-FITC was the secondary antibody used for this test.
      Shaded histogram represents cells without antibody; green represents isotype control; red represents anti CD14 antibody.

    References

    This product has been referenced in:
    • Brito BE  et al. Toll-like receptor 4 and CD14 expression in human ciliary body and TLR-4 in human iris endothelial cells. Exp Eye Res 79:203-8 (2004). Read more (PubMed: 15325567) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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