Anti-CD14 antibody [UCHM1] (ab6623)

Overview

  • Product name
    Anti-CD14 antibody [UCHM1]
    See all CD14 primary antibodies
  • Description
    Mouse monoclonal [UCHM1] to CD14
  • Specificity
    ab6623 recognises a monocyte surface antigen of 55 kDa found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens. Oxford 1986, as CDw14, group A. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Human Thymocytes followed by peripheral blood mononuclear cells

  • Positive control
    • Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab6623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr 1/10.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
    • Tissue specificity
      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
    • Sequence similarities
      Contains 11 LRR (leucine-rich) repeats.
    • Post-translational
      modifications
      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • ab6623 staining of human peripheral blood monocytes, visualised with rabbit anti-mouse IgG: FITC.

    References

    ab6623 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This antibody has been tested on paraffin sections and does not work. In IHC, it is known to work only with frozen sections.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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