Anti-CD146 antibody [c264] (Phycoerythrin) (ab25435)

Overview

  • Product nameAnti-CD146 antibody [c264] (Phycoerythrin)
    See all CD146 primary antibodies
  • Description
    Mouse monoclonal [c264] to CD146 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25435 recognise Chicken MCAM, Mr 98 kDa (protein core ˜84 kDa).
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Pooled Ed15 and Ed16 thymocytes from H.B15, H.B19 and H.B21 chicken embryos

Properties

Applications

Our Abpromise guarantee covers the use of ab25435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionPlays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
  • Tissue specificityDetected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • A32 antigen antibody
    • CD 146 antibody
    • CD146 antibody
    • CD146 antigen antibody
    • Cell surface glycoprotein MUC18 antibody
    • Cell surface glycoprotein P1H12 antibody
    • Gicerin antibody
    • Mcam antibody
    • Melanoma adhesion molecule antibody
    • Melanoma associated antigen A32 antibody
    • Melanoma associated antigen MUC18 antibody
    • Melanoma associated glycoprotein MUC18 antibody
    • Melanoma cell adhesion molecule antibody
    • Melanoma-associated antigen A32 antibody
    • Melanoma-associated antigen MUC18 antibody
    • MelCAM antibody
    • MUC 18 antibody
    • MUC18 antibody
    • MUC18_HUMAN antibody
    • S endo 1 antibody
    • S endo 1 endothelial associated antigen antibody
    • S-endo 1 endothelial-associated antigen antibody
    see all

Anti-CD146 antibody [c264] (Phycoerythrin) images

  • Flow cytometry analysis staining CD146 in chicken thymocytes using ab25435 at a dilution of 0.1 ug/106 cells. Mouse anti-chicken MCAM-PE was used as an isotype control.

References for Anti-CD146 antibody [c264] (Phycoerythrin) (ab25435)

ab25435 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"