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Anti-CD147 antibody [AB1843] (ab49493)

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Overview

Product name

Anti-CD147 antibody [AB1843]
See all CD147 products (31) ...

Description

Mouse monoclonal [AB1843] to CD147

Tested applications

WB, IHC-P, Blockingmore details

Cross reactivity

Reacts with

Human

Immunogen

Prokaryotic recombinant fusion protein corresponding to a 147 amino acid region of the extracellular domain of the human CD147 molecule

Positive control

Human skin (IHC), SAOS-2 cell line (WB).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

AB1843

Myeloma

P3-NS1/1-Ag4-1

Isotype

IgG1

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Sensory System >> Visual system
Immunology >> Cell Type Markers >> CD >> Endothelial Cells

  • Immunohistochemistry (Paraffin-embedded sections) - CD147 antibody [AB1843] (ab49493)Immunohistochemistry (Paraffin-embedded sections) - CD147 antibody [AB1843] (ab49493) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/100Predicted molec...Read more →

    WB: 1/100Predicted molecular weight: 42 kDa.

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/20 - 1/40.Perfo...Read more →

    IHC-P: 1/20 - 1/40.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ShowHide

    Blocking

     BL: Use at an assay depe...Read more →

    BL: Use at an assay dependent concentration. (PubMed: 20979056)

Application notes

Is unsuitable for or IHC-Fr.

Target

Function

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

Tissue specificity

Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Post-translational
modifications

N-glycosylated.

Cellular localization

Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP35613 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 5A11 antigen antibody
  • 5F7 antibody
  • BASI_HUMAN antibody
  • Basigin (Ok blood group) antibody
  • Basigin antibody
  • Blood brain barrier HT7 antigen antibody
  • BSG antibody
  • CD 147 antibody
  • CD147 antibody
  • CD147 antigen antibody
  • Collagenase stimulatory factor antibody
  • Emmprin antibody
  • Extracellular matrix metalloproteinase inducer antibody
  • Leukocyte activation antigen M6 antibody
  • M 6 antibody
  • M6 antibody
  • M6 antigen antibody
  • M6 leukocyte activation antigen antibody
  • Neurothelin antibody
  • OK antibody
  • OK blood group antibody
  • OK blood group antigen antibody
  • TCSF antibody
  • Tumor cell derived collagenase stimulatory factor antibody
  • Tumor cell-derived collagenase stimulatory factor antibody
see all

Database links

Anti-CD147 antibody [AB1843] images:

  Immunohistochemistry (Paraffin-embedded sections) - CD147 antibody [AB1843] (ab49493)

Immunohistochemistry (Paraffin-embedded sections) - CD147 antibody [AB1843] (ab49493)

Ab49493 at 1/20 dilution staining malignant cells of Human lung adenocarcinoma; paraffin embedded; used high temperature antigen unmasking technique.

References for Anti-CD147 antibody [AB1843] (ab49493)

This product has been referenced in:

  • Kornek Met al. Human T cell microparticles circulate in blood of hepatitis patients and induce fibrolytic activation of hepatic stellate cells. Hepatology 53:230-42 (2011). Blocking; Human.Read more (PubMed: 20979056) »
  • Kuang YHet al. RNA interference targeting the CD147 induces apoptosis of multi-drug resistant cancer cells related to XIAP depletion. Cancer Lett 276:189-95 (2009). WB; Human.Read more (PubMed: 19097686) »

See all 2 publications for this product

Publishing research using ab49493? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"