Overview

  • Product nameAnti-CD147 antibody
    See all CD147 primary antibodies
  • Description
    Mouse polyclonal to CD147
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CD147 protein (NP_940991.1)

  • Positive control
    • WB: Human spleen tissue lysate and CD147 transfected 293T cell lysate IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all

Anti-CD147 antibody images

  • ab88530, at 10 µg/ml staining CD147 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
  • Anti-CD147 antibody (ab88530) at 1 µg/ml + Human spleen tissue lysate at 50 µg

    Predicted band size : 42 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD147 antibody (ab88530) at 1 µg/ml

    Lane 1 : CD147 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-CD147 antibody (ab88530)

This product has been referenced in:
  • Sandvig K & Llorente A Proteomic analysis of microvesicles released by the human prostate cancer cell line PC-3. Mol Cell Proteomics 11:M111.012914 (2012). WB ; Human . Read more (PubMed: 22457534) »

See 1 Publication for this product

Product Wall

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The immunogen is BSG (NP_940991, 1 a.a. ˜ 269 a.a) full-length human protein. The expression system is mammalian cell, and post modification does exist in the system.
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Unfortunately we do not carry small aliquots of our antibodies. Since we have over 70,000 products, logistically we cannot store multiple vial sizes of the same product.


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