Recombinant
RabMAb

Anti-CD147 antibody [EPR18008-67] (ab212057)

Overview

  • Product name
    Anti-CD147 antibody [EPR18008-67]
    See all CD147 primary antibodies
  • Description
    Rabbit monoclonal [EPR18008-67] to CD147
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD147 aa 100-350. The exact sequence is proprietary.
    Database link: P18572

  • Positive control
    • WB: His-tagged mouse CD147 recombinant protein fragment (aa140-325); RAW 264.7, WEHI-3 and bEND.3 whole cell lysates; Mouse liver lysate. IHC-P: Mouse intestine tissue. ICC/IF: bEnd.3 and WEHI-231 cells. Flow Cyt: Mouse thymocytes. IP: RAW 264.7 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab212057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.
Flow Cyt 1/200.
IP 1/50.

Target

  • Function
    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificity
    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all

Images

  • Anti-CD147 antibody [EPR18008-67] (ab212057) at 1/5000 dilution + His-tagged mouse CD147 recombinant protein fragment (aa140-325) at 0.002 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking and dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-CD147 antibody [EPR18008-67] (ab212057) at 1/5000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : WEHI-3 (mouse leukemia cell line) whole cell lysate
    Lane 4 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa
    Observed band size : 45-55 kDa (why is the actual band size different from the predicted?)

     

    Exposure time : Lanes 1/2: 3 minutes; Lane 3: 10 seconds; Lane 4: 1 second.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16721788; PMID: 23966157).

  • Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling CD147 with ab212057 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on mouse intestine is observed. Counter stained withematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelioma cell line) cells labeling CD147 with ab212057 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membranous staining on bEnd.3 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative control are is as follows:

    -ve control 1: ab212057 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

     

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse lymphoblast B cell lymphoma cell line) cells labeling CD147 with ab212057 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membranous staining on bEnd.3 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative control are is as follows:

    -ve control 1: ab212057 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow cytometric analysis of fresh mouse thymocytes labeling CD147 with ab212057 at 1/200 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/500 dilution was used as the secondary antibody.

  • CD147 was immunoprecipitated from 1 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab212057 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab212057 at 1/5000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate 10μg (Input).

    Lane 2: ab212057 IP in RAW 264.7 whole cell lysate.

    Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab212057 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab212057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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