Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)

Overview

  • Product nameAnti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free
    See all CD147 primary antibodies
  • Description
    Mouse monoclonal [MEM-M6/6] to CD147 - Low endotoxin, Azide free
  • Specificityab119114 recognizes Ig domain D2 (membrane proximal) of CD147(Neurothelin).
  • Tested applicationsSuitable for: ICC/IF, WB, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Protein A-CR purified soluble recombinant Human CD147 (consisting of the CDNA encoding the hinge region, CH2-and CH3 domain of Human IgG1).

  • Positive control
    • 293 Human fibroblastoid cell line.
  • General notesEndotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Properties

Applications

Our Abpromise guarantee covers the use of ab119114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 42 kDa.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all

Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free images

  • Human peripheral blood lymphocytes stained with ab119114 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab119114, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • ICC/IF image of ab119114 stained SV40 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119114, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD147 antibody [MEM-M6/6] - Low endotoxin, Azide free (ab119114)

ab119114 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

The following paper describes production and characterization of the MEM-M6/6 clone, apparently by the same laboratory that wrote the 2003 paper from which you obtained your protocol.

Koch C. et al. T ...

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Thank you for contacting Abcam regarding these antibodies. I have spoken with our laboratory regarding the light chain isotypes of ab666 and ab119114. Unfortunately this information is not available. Please do not hesitate to contact us if ther...

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Vielen Dank für Ihre Anfrage. Leider haben wir keinen JAM-A (Junctional Adhesion Molecule 1), der auch für funktionale Studien getestet ist. Ich möchte Ihnen deshalb die Webseite Biocompare empfehlen. http://www.biocompare.com/ Für EMMPRIN ...

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