Overview

  • Product nameAnti-CD147 antibody [OX114]
    See all CD147 primary antibodies
  • Description
    Rat monoclonal [OX114] to CD147
  • SpecificityThis antibody recognises both isoforms of CD147.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein mCD147dO-2rCD4d3 +4

  • Positive control
    • mouse splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab34016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/25 - 1/50. Use 10µl of the suggested working dilution to label 1 x 106 cells. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.



ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.

Target

  • FunctionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificityPresent only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationCell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5A11 antigen antibody
    • 5F7 antibody
    • BASI_HUMAN antibody
    • Basigin (Ok blood group) antibody
    • Basigin antibody
    • Blood brain barrier HT7 antigen antibody
    • Bsg antibody
    • CD 147 antibody
    • CD147 antibody
    • CD147 antigen antibody
    • Collagenase stimulatory factor antibody
    • EMMPRIN antibody
    • Extracellular matrix metalloproteinase inducer antibody
    • Leukocyte activation antigen M6 antibody
    • M 6 antibody
    • M6 antibody
    • M6 leukocyte activation antigen antibody
    • Neurothelin antibody
    • OK antibody
    • OK blood group antibody
    • OK blood group antigen antibody
    • TCSF antibody
    • Tumor cell derived collagenase stimulatory factor antibody
    • Tumor cell-derived collagenase stimulatory factor antibody
    see all

Anti-CD147 antibody [OX114] images

  • ab34016 staining CD147 in mouse peripheral blood lymphocytes by Flow Cytometric analysis.

References for Anti-CD147 antibody [OX114] (ab34016)

This product has been referenced in:
  • Seizer P  et al. Cyclophilin A affects inflammation, virus elimination and myocardial fibrosis in coxsackievirus B3-induced myocarditis. J Mol Cell Cardiol 53:6-14 (2012). IHC ; Mouse . Read more (PubMed: 22446162) »
  • Kato N  et al. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J Am Soc Nephrol 20:1565-76 (2009). Read more (PubMed: 19443639) »

See all 3 Publications for this product

Product Wall


The epitope that ab108317 recognizes is extracellular, within the range of amino acids from 175 - 200 of human CD147, UniProt P35613. http://www.uniprot.org/uniprot/P35613.

The immunogen for the rat monoclonal [OX114], ab34016, and mapp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"